Figure 4

Graphical displays of BCP hot-spot results from the SCIDX1, CGD, and X-linked ALD trials. Our hot-spot software produces three types of plots for viewing hot-spot results, (A) a stripchart that displays the full genome for all data sets analyzed, (B) a stripchart that displays results for all data sets, one chromosome at a time; and (C) a barplot that displays results for one data set and chromosome at a time. In all plot types the grey color corresponds to VIS (A, B) or VIS bins (C) that were not defined as hot-spots. In all three plot types the x-axis corresponds to location in megabase units. In plot type C, the y-axis corresponds to bin rate (# VIS per bin/total # VIS) rather than z-score for visual clarity since z-scores can be negative. Color definitions were assigned to each data set independently based on quantiles of its non-zero z-score distribution (ie, the distribution of bin z-scores among bins with non-negative scores). VIS that were located in hot-spot regions corresponding to bins with z-score distributions ≤ 85th percentile are colored light blue, > 85 and ≤ 95 are dark blue, > 95 and ≤ 97.5 are purple, > 97.5 and ≤99 are pink and > 99 are colored red. The plots illustrate hot-spots on chromosomes 6, 11, 12 and 17 in the X-linked ALD data set, and the presence of the chromosome 6 hot-spot in both patients analyzed separately. The MLV data sets exhibit unique VIS patterns that differ from each other as well as the LV data.