Figure 1
From: Toponomics method for the automated quantification of membrane protein translocation
Structure identification. A. Representative canaliculus suitable for intensity profile extraction. It is straight and uniform, tight junctions are symmetrical, undisrupted and run in parallel. Protein intensity profiles of a length 8 μ m are extracted by measuring the pixel intensities of two channels (Bsep, red, functional marker protein; Zo-1, green, structural marker protein) perpendicular to the canaliculus. For example, one of the possibly extracted profiles is shown by a white line. B. Canalicular regions are extracted using the foreground-background detection function of the Zeta software [21]. Foreground mask of the canaliculus shown in A. White areas are considered for further processing. C. Skeletonization results.