Averaged fluorescence intensity profiles with standard deviations. Data is shown for the first image of dataset K1 (Bsep, functional marker protein - red; Zo-1, structural marker protein - green). A. All Zo-1 profiles (n = 4166). B. All centered Zo-1 profiles (n = 4166). C. Accepted Zo-1 profiles after the selection procedure (n = 46). D. All Bsep profiles. E. All centered Bsep profiles. F. Accepted Bsep profiles.