|
Nucleotide
|
A
|
C
|
T
|
G
|
Total f
|
Per cycle p
|
|---|
|
A
|
0.9995
|
7.2e-6
|
7.7e-6
|
5.1e-4
|
5.2e-4
|
3.5e-5
|
|
C
|
1.1e-05
|
0.9996
|
4.1e-4
|
2.1e-6
|
4.2e-4
|
2.8e-5
|
|
T
|
9.0e-6
|
5.7e-4
|
0.9994
|
1.4e-5
|
5.9e-4
|
4.0e-5
|
|
G
|
3.5e-4
|
3.2e-6
|
2.1e-5
|
0.9996
|
3.7e-4
|
2.5e-5
|
- The rows give the true nucleotides and the columns the observed as a result of errors. Estimates of the individual probabilities are given as frequencies. The total error probability f for each nucleotide is also quoted in the penultimate column, followed by the per cycle error rate p calculated as p = 2f /n, where n = 30 is the cycle number [8, 17].
