Figure 5

Spoke represented complex. This figure shows a possible n-ary interaction (six proteins in this case) represented using a spoke model where a series of binary interactions between one member chosen to represent the hub and each of the other members are created (see section 4.1 for more details). The "Show spoke-represented complexes" function in the iRefScape plug-in was used to identify such disassembled n-ary interactions and visually show the connection using a pseudonode (the gray hexagon in the figure) and a set of dashed edges. In the figure, the original five binary interactions from source databases are shown as continuous yellow edges between the six proteins. The data is from an affinity chromatography experiment [27] and is represented in both BioGRID and IntAct using a spoke-model. The white box indicates three subunits that overlap with the five genes encoding subunits of the translation initiation factor EIF2B. Mutations in any one of these subunits are associated with Leukoencephalopathy (OMIM #603896). The overlap between the Leukoencephalopathy gene list and the list of genes in this regenerated n-ary record are significant; however, the two lists would not normally be compared because the n-ary record appears as a series of binary records in interaction databases.