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Table 1 Statistical results for univariate analysis

From: pcrEfficiency: a Web tool for PCR amplification efficiency prediction

Univariate analysis  
Variable value df ρ d Log Odds p-value
Primers length Z = -7.4398 - -0.118 -0.239 -0.433 1.008e-13*
Sequence length Z = -5.423 - -0.086 -0.173 -0.314 5.86e-08*
Sequence G+C content Z = -10.2664 - -0.163 -0.331 -0.601 <2.2e-16*
A repeats Z = 2.1004 - 0.033 0.067 0.121 0.03569*
T repeats Z = 3.9818 - 0.063 0.127 0.230 6.84e-05*
C repeats Z = -5.294 - -0.084 -0.169 -0.307 1.196e-07*
G repeats Z = -7.1808 - -0.114 -0.230 -0.418 6.929e-13*
Primers T m Z = 1.4653 - 0.023 0.047 0.085 0.1428
Primers self complementarity Z = 11.9002 - 0.190 0.386 0.700 <2.2e-16*
Primer dimers Z = 4.4161 - 0.070 0.141 0.256 1.005e-05*
Primer GC imbalance Z = 11.1367 - 0.177 0.360 0.654 <2.2e-16*
Primers GC content Z = 4.5921 - 0.073 0.147 0.266 4.388e-06*
Sequence palindromes Z = -3.4951 - -0.056 -0.111 -0.202 0.0004738*
Species χ2 = 585.616 9 - - - <2.2e-16*
Template χ 2 = 1241.562 12 - - - <2.2e-16*
Variety or line χ 2 = 585.8386 18 - - - <2.2e-16*
Template source χ 2 = 940.8915 24 - - - <2.2e-16*
Operator χ 2 = 727.4887 8 - - - <2.2e-16*
Primer's 3' last two nucleotides χ 2 = 237.911 15 - - - <2.2e-16*
  1. In the case of asymptotic Spearman tests, Z value are shown; χ2 value is written for asymptotic Kruskal-Wallis tests. An asterisk over the p-value reflects a significant influence (p-value ≤ 0.05). Sperman's correlation analysis shows the ρ statistic as well the Cohen's d and Log Odds estimates of effect size. For post-hoc asymptotic Wilcoxon Mann-Whitney rank sum tests and its effect size estimators see Additional File 2.