Table 1 Statistical results for univariate analysis

Primers length

Z = -7.4398

-

-0.118

-0.239

-0.433

1.008e-13*

Sequence length

Z = -5.423

-

-0.086

-0.173

-0.314

5.86e-08*

Sequence G+C content

Z = -10.2664

-

-0.163

-0.331

-0.601

<2.2e-16*

A repeats

Z = 2.1004

-

0.033

0.067

0.121

0.03569*

T repeats

Z = 3.9818

-

0.063

0.127

0.230

6.84e-05*

C repeats

Z = -5.294

-

-0.084

-0.169

-0.307

1.196e-07*

G repeats

Z = -7.1808

-

-0.114

-0.230

-0.418

6.929e-13*

Primers T _m

Z = 1.4653

-

0.023

0.047

0.085

0.1428

Primers self complementarity

Z = 11.9002

-

0.190

0.386

0.700

<2.2e-16*

Primer dimers

Z = 4.4161

-

0.070

0.141

0.256

1.005e-05*

Primer GC imbalance

Z = 11.1367

-

0.177

0.360

0.654

<2.2e-16*

Primers GC content

Z = 4.5921

-

0.073

0.147

0.266

4.388e-06*

Sequence palindromes

Z = -3.4951

-

-0.056

-0.111

-0.202

0.0004738*

Species

χ² = 585.616

9

-

-

-

<2.2e-16*

Template

χ ² = 1241.562

12

-

-

-

<2.2e-16*

Variety or line

χ ² = 585.8386

18

-

-

-

<2.2e-16*

Template source

χ ² = 940.8915

24

-

-

-

<2.2e-16*

Operator

χ ² = 727.4887

8

-

-

-

<2.2e-16*

Primer's 3' last two nucleotides

χ ² = 237.911

15

-

-

-

<2.2e-16*