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Figure 3 | BMC Bioinformatics

Figure 3

From: Dual channel rank-based intensity weighting for quantitative co-localization of microscopy images

Figure 3

Effect of non-co-occurring pixels on correlation. Sets of synthetic dual channel images with varying levels of co-occurrence were generated with all the co-occurring pixels in the image having a correlation of R = 1.00, and all the non-occurring pixels in the image having a correlation of R≈0.00. (A) Example showing the presence of 20% co-occurring pixels, resulting in an overall correlation of R = 0.12 for the entire image. (B) Example showing the presence of 40% co-occurring pixels, resulting in an overall correlation of R = 0.18 for the entire image. (C) Example showing the presence of 60% co-occurring pixels, resulting in an overall correlation of R = 0.26 for the entire image. (D) Example showing the presence of 100% co-occurring pixels, resulting in an overall correlation of R = 1.00 for the entire image. This demonstrates the importance of using only the co-occurring pixels for quantifying correlation and co-localization.

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