From: Empirical comparison of cross-platform normalization methods for gene expression data
Manufacture | Platform | Probe Length | Probe Type | Probe Construction | Number of Probes | Label Type | Detection method |
---|---|---|---|---|---|---|---|
Applied Biosystems | Human Genome Survey Microarray v2.0 | 60 | DNA oligonucleotide with 3' carbon spacer to reduce steric effects | Presynthesized and contact spotted | 32878 | Digoxigenin (DIG) UTP | anti-DIG phosphatase catalyzed Chemiluminescence |
Affymetrix | HG-U133 Plus 2.0 GeneChip® | 25 | DNA oligonucleotide | In situ photolithography | 54675 | Biotin | phycoerythrin-streptavidin-antibody fluorescence |
Agilent | Whole Human Genome Oligo Microarray, G4112A | 60 | DNA oligonucleotide | In situ inkjet printing | 43931 | Cy3 or Cy5 | Cy3 or Cy5 fluorescence |
Illumina | Human-6 BeadChip, 48K v1.0 | 50 | DNA oligonucleotide with 29 base address sequence as linker | Presynthesized, immobilized on beads, and randomly deposited in wells | 47293 | Biotin | strepatavidin-Cy3 fluorescence |