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Table 1 Characteristics of relevant microarray platforms

From: Empirical comparison of cross-platform normalization methods for gene expression data

Manufacture

Platform

Probe Length

Probe Type

Probe Construction

Number of Probes

Label Type

Detection method

Applied Biosystems

Human Genome Survey Microarray v2.0

60

DNA oligonucleotide with 3' carbon spacer to reduce steric effects

Presynthesized and contact spotted

32878

Digoxigenin (DIG) UTP

anti-DIG phosphatase catalyzed Chemiluminescence

Affymetrix

HG-U133 Plus 2.0 GeneChip®

25

DNA oligonucleotide

In situ photolithography

54675

Biotin

phycoerythrin-streptavidin-antibody fluorescence

Agilent

Whole Human Genome Oligo Microarray, G4112A

60

DNA oligonucleotide

In situ inkjet printing

43931

Cy3 or Cy5

Cy3 or Cy5 fluorescence

Illumina

Human-6 BeadChip, 48K v1.0

50

DNA oligonucleotide with 29 base address sequence as linker

Presynthesized, immobilized on beads, and randomly deposited in wells

47293

Biotin

strepatavidin-Cy3 fluorescence

  1. Characteristics of microarray platforms analyzed in this work. See references [7–10] for information sources.