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Table 3 Quince's 454 data

From: Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows

 

Artificial

Priest Pot

 

3%

5%

10%

3%

5%

10%

CROP

41

25

15

562

246

42

ESPRIT

248

77

38

1115

773

394

MAFFT+JC

686

686

686

3764

3764

3764

MAFFT+MAFFT

31933

31933

31933

15984

15984

15984

MAFFT+Mothur

1756

1756

1756

6672

6672

6672

Mothur+JC

49

36

33

640

537

537

Mothur+MAFFT

4276

4276

4276

2824

2824

2824

Mothur+Mothur

113

53

53

766

642

642

Mothur+PreC+ JC

61

40

36

662

482

482

Mothur+PreC+MAFFT

3864

3141

3141

1905

1625

1625

Mothur+PreC+Mothur

136

65

46

810

575

575

MUSCLE+JC

146

146

146

4059

4059

4059

MUSCLE+MAFFT

33491

33491

33491

15718

15718

15718

MUSCLE+Mothur

258

258

258

6433

6433

6433

Otupipe

66

39

24

793

570

302

RDP

250

94

43

1209

862

456

  1. OTUs observed with each of the workflows analysed at distances of 3, 5 and 10% for Quince's Artificial and Priest Pot datasets. For the Priest Pot data, 855 OTUs at 3% and 699 at 5% were previously estimated by Quince et al. [26].