Skip to main content

Table 5 Near-full length sequences

From: Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows

  Skin axillary Prairie soil
  3% 5% 10% 3% 5% 10%
CROP 1009 1009 1009 1128 1128 1128
ESPRIT 59 43 26 504 340 162
MAFFT+JC 47 39 39 490 346 183
MAFFT+MAFFT 266 126 109 1007 895 714
MAFFT+Mothur 50 39 36 503 351 181
Mothur+JC 47 39 36 491 352 184
Mothur+MAFFT 269 126 109 977 872 689
Mothur+Mothur 54 40 38 535 396 204
Mothur+PreC+ JC 47 39 36 491 353 268
Mothur+PreC+MAFFT 287 127 109 977 877 836
Mothur+PreC+Mothur 55 40 38 536 397 305
MUSCLE+JC 50 39 37 480 337 175
MUSCLE+MAFFT 266 126 109 1007 895 714
MUSCLE+Mothur 58 40 38 487 339 173
Otupipe 49 37 26 490 336 160
RDP 60 43 27 504 348 175
  1. OTUs observed with each of the workflows analysed at distances of 3, 5 and 10% for the skin axillary microbiome data described by Hao et al. [23], and tall grass prairie soil data [37].