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Table 7 Observed output variability

From: Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows

   50 × 60 seqs mut stacked 50 × 60 seqs mut interleaved Prairie soil
  N μ Err Min Max μ Err Min Max μ Err Min Max
CROP 3% 20 1852.2 2.16 1847.6 1856.7 1954.6 0.83 1952.8 1956.3 1128 0 1128 1128
CROP 5% 20 1853.5 2.43 1848.4 1858.5 1952.4 0.66 1951 1953.7 1128 0 1128 1128
CROP 10% 20 1850.9 1.65 1847.4 1854.4 1951.7 0.62 1950.4 1953 1128 0 1128 1128
ESPRIT 3% 20 205 0 205 205 193 0 193 193 565 0 565 565
ESPRIT 5% 20 59 0 59 59 59 0 59 59 381 0 381 381
ESPRIT 10% 20 56 0 56 56 56 0 56 56 180 0 180 180
Mothur 3% 20 1465.3 0.23 1464.8 1465.8 1173 0 1173 1173 541 0 541 541
Mothur 5% 20 1465.3 0.23 1464.8 1465.8 1173 0 1173 1173 400 0 400 400
Mothur 10% 20 1465.3 0.23 1464.8 1465.8 1173 0 1173 1173 347 0 347 347
Mothur+PreC 3% 20 1490.6 0.16 1490.3 1490.9 1197.3 0.12 1197 1197.6 541 0 541 541
Mothur+PreC 5% 20 1490.6 0.16 1490.3 1490.9 1197.3 0.12 1197 1197.6 400 0 400 400
Mothur+PreC 10% 20 1490.6 0.16 1490.3 1490.9 1197.3 0.12 1197 1197.6 348 0 348 348
  1. Observed output variability with 20 equal runs of CROP, ESPRIT and Mothur (with (PreC) and without preclustering). N is the number of observations for all samples; μ is the observed mean of the sample. Standard error (Err) and confidence intervals (Min and Max) were calculated from these values, as described in Methods. Many of the CROP calculations failed to complete in this test, as reflected by N (Prairie soil), and statistics were adjusted accordingly.