BMC Bioinformatics

Table 7 Observed output variability

From: Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows

		50 × 60 seqs mut stacked				50 × 60 seqs mut interleaved				Prairie soil
	N	μ	Err	Min	Max	μ	Err	Min	Max	μ	Err	Min	Max
CROP 3%	20	1852.2	2.16	1847.6	1856.7	1954.6	0.83	1952.8	1956.3	1128	0	1128	1128
CROP 5%	20	1853.5	2.43	1848.4	1858.5	1952.4	0.66	1951	1953.7	1128	0	1128	1128
CROP 10%	20	1850.9	1.65	1847.4	1854.4	1951.7	0.62	1950.4	1953	1128	0	1128	1128
ESPRIT 3%	20	205	0	205	205	193	0	193	193	565	0	565	565
ESPRIT 5%	20	59	0	59	59	59	0	59	59	381	0	381	381
ESPRIT 10%	20	56	0	56	56	56	0	56	56	180	0	180	180
Mothur 3%	20	1465.3	0.23	1464.8	1465.8	1173	0	1173	1173	541	0	541	541
Mothur 5%	20	1465.3	0.23	1464.8	1465.8	1173	0	1173	1173	400	0	400	400
Mothur 10%	20	1465.3	0.23	1464.8	1465.8	1173	0	1173	1173	347	0	347	347
Mothur+PreC 3%	20	1490.6	0.16	1490.3	1490.9	1197.3	0.12	1197	1197.6	541	0	541	541
Mothur+PreC 5%	20	1490.6	0.16	1490.3	1490.9	1197.3	0.12	1197	1197.6	400	0	400	400
Mothur+PreC 10%	20	1490.6	0.16	1490.3	1490.9	1197.3	0.12	1197	1197.6	348	0	348	348

Observed output variability with 20 equal runs of CROP, ESPRIT and Mothur (with (PreC) and without preclustering). N is the number of observations for all samples; μ is the observed mean of the sample. Standard error (Err) and confidence intervals (Min and Max) were calculated from these values, as described in Methods. Many of the CROP calculations failed to complete in this test, as reflected by N (Prairie soil), and statistics were adjusted accordingly.

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