Targets of Gli in the hedgehog pathway and motif alignment of a highly conserved Gli1 binding site. Network showing the known Gli targets in mouse. All direct targets were recovered by MotifMap, including the autoregulatory loop of Gli1. Nkx2-2, Nkx2-8 and Ptch2 are examples of additional direct targets identified by MotifMap with binding site conserved in more than 25 out of the 30 species in the genome alignment. (5b) Motif alignment for a highly conserved Gli1 binding site Motif alignment for a highly conserved Gli1 binding site 1365 bp upstream of the Nkx2-8 transcription start site is also shown.