Figure 5

Nkx2.2 binding to the NeuroD1 promoter through GAGT and AAGT sites. (A) Schematic representation of the NeuroD1 promoter. The TRANSFAC PWM predicted two sites that were not bound in vitro or in vivo [20]. PBM-mapping predicted a novel site upstream of the other two sites that contains both a GAGT core and an AAGT core separated by 4 bp. (B) EMSA analysis showing binding through both core sites. Wildtype, AAGT mutant, GAGT mutant and double mutant probes were incubated with in vitro translated Nkx2.2.