Figure 6

Box plots of log fold changes. We investigate the distribution of the log fold change of genes identified as significantly up- regulated at the optimal hybridization temperature but lost when hybridizing one degree Celsius too high. The left box plot shows that genes with sufficiently large fold change could also be detected in hybridizations at 52°C. The right box plot contrasts this with the distribution for genes only identified as up-regulated at the optimal hybridization temperature. Genes with more subtle fold changes were therefore lost at the suboptimal hybridization temperature. This particularly affects small copy number molecules, including many regulators.