Figure 8From: ATAQS: A computational software tool for high throughput transition optimization and validation for selected reaction monitoring mass spectrometryIdentified kinase peptide transitions in a cancer cell line. (a) shows the smoothed heavy and light transitions of the kinase peptide AQLSTILEEEK in three different heavy peptide-spiked samples. (b) shows the kinase peptide IISIFSGTEK transitions. (a) and (b) show how ATAQS calculates the properties described in the 'identification of confirmed transitions' guided peptide detection in the sample, in spite of the strong interference. (c) shows an example of a decoy peptide RNVSTESIEF, which is above the FDR score cutoff of <1.2% in validation.Back to article page