Figure 1From: High-throughput peptide quantification using mTRAQ reagent triplexExamples of overlapping triplex isotopic clusters. (a) Schematic model of overlapping triplex isotopic clusters. Blue, red, and green lines represent isotopic peaks of light-, medium-, and heavy-labeled peptides, respectively. (b) Experimental overlapping isotopic clusters of ‘EPMIGVNQELAYFYPELFR’. We can observe that the first isotopic peaks of medium- and heavy-labeled peptides are clearly higher than the corresponding third peaks, and it is evidence for the existence of overlaps.Back to article page