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Figure 2 | BMC Bioinformatics

Figure 2

From: High-throughput peptide quantification using mTRAQ reagent triplex

Figure 2

Elution area approximation to normal distribution. Elution area approximation for ‘HPIKHQGLPQEVLNENLLR’. The blue line represents the sum of intensities of the peptide over the elution area and the red line is an approximated normal distribution. From the given elution time (951.423 s), where tandem MS scan was acquired, we first found the maximum point of elution area (957.93 s). Then we extended the area until the sum of intensities is below 50% of that of the maximum point and obtained μ = 960.63 and FWHM = 19.9. Finally, we used the area with higher intensities than 10% of the maximum intensity of the approximated normal distribution.

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