Figure 4
From: High-throughput peptide quantification using mTRAQ reagent triplex
Manual inspection for the peptides whose computed ratios are different from the expected ratio. a) The most abundant isotopic clusters for ‘DMPIQAFLLYQEPVLGPVRGPFPIIV’. The red lines represent its theoretical isotopic distribution. The expected ratio is 5:10:1, and our algorithm computed 5.612575 as M/L and 0.163601 as H/L for this peptide. (b) The most abundant isotopic clusters for ‘ALNEINQFYQK’. The expected ratio is 1:1:3, and our algorithm computed 2.074808 as M/L and 1.48508 as H/L. It is clear that our ratios are more suitable than the expected ratios for these isotopic clusters.