Fig. 6

The NTD Kit Components. (Upper Left) One inch diameter Teflon core machined to contain two chambers that are connected by a segment of Teflon tubing. This tubing has one end open while the other is capped to restrict it’s opening to a tiny pin hole (Aperture) approximately 25 microns in diameter (not shown). (Upper Right) Aluminum square block is machined to support the core and provide access for illumination and electrode ports, and have easy assembly and mounting on the Peltier device. (Lower Left) The Teflon and aluminum components are shown seated on the Peltier device for temperature control of the wells. Silver chloride (Ag/AgCl) pellet electrodes are shown inserted into the cis and trans chambers port wells. (Lower Right) A NTD Nanoscope kit arrangement that uses a table-top vibration isolation table, has perfusion apparatus, Faraday cage, and linkage to a computer (to left of workstation, not shown).