Fig. 8

The Y-SNP test complex with 35 dT length overhang is shown at the base-level specification, where a SNP base is as shown. If the SNP is its variant form (typically only one other base possibility is common), then a base-pairing will not occur at the nexus of the Y-SNP shown. This allows discrimination between the annealed forms with high accuracy, while also discerning from the signals produced by the non-annealed Y-SNP, where there is no target-bound, or only non-specific molecular interactions imparting much less conformational structure as occurs with the matching (or mostly matching) annealing interaction.