Comparison of RMSF with crystal template with built model. Red circle indicated high fluctuation. Higher fluctuation was seen in Ser47 as compared to the crystal structure (template) due to the fact that Ser47 extruded out from the lipid bilayer and exposed to the water. The coil regions such as residues 97-102, 129-138, 212-215 and the C terminal of chain D in the build model had showed also the high fluctuation as compare the helices in the structure. There are two missing residues at residues 119-120 in the crystal structure which cause the sudden decrease of RMSF occurred.