Full-length genes reconstructed by each method at different expression quintiles. Shown are the percentages of reconstructed full-length genes (Y axis) at different expression quintiles (X axis, 10% increment) by Oases with different k-mer values using Dme-3g (a) and Spo-1g (b) or by each assembler using Dme-3g (c) and Dme-13g (d) data sets. (e) An example is shown as an assembled transcript in D. melanogaster by different assembly methods. NM_079795 is one of the highly expressed genes at highest expression quintile, which could be completely reconstructed by Trinity (red), but failed by other methods. Only incomplete transcripts (green) were reconstructed and both ends of coding region were lost. Incomplete transcript with 1 bp deletion assembled by Oases-MK is shown below its gene model. Reads coverage is shown at the bottom.