Figure 2

Peptide Microarray Evaluation Datasets. Peptides selected to bind known epitope monoclonals (A) or anti-peptide polyclonal sera (B) were used to predict the epitope (A) or immunizing peptide (B) in GuiTope, RELIC, or Glam2 within a database of decoy sequences. The significance scores of the true epitope or immunizing peptide sequences was compared to the decoy sequences using ROC plots, where the true positive rate is plotted against the false positive rate for all possible score thresholds. The results using the inversion weight as one are plotted in blue, the results without inversions are plotted in red, the results for RELIC are plotted in green, and the results for Glam2 are plotted in black. The AUROC value shown in the legend indicates the probability that a true sequence would score higher than a decoy sequence for that dataset. *Note that the RELIC analysis of the monoclonal set is only based on five monoclonal antibodies because the sixth antibody was run after the server was no longer available.