Figure 2

Synthetic Populations used in Testing. Synthetic data were generated to measure the performance of the three different methods in a case where 'ground truth' is known. Samples were randomly drawn from a low abundance population (Low, blue line), high abundance population (High, red line) or a WT population (WT, black line) as shown in the upper panels (A, C). Two population structures were sampled, one with a low probability of WT, P(WT = 0.4), and the other with a high probability of WT, P(WT) = 0.93, shown in the lower panels (B, C). To test the effect of population shape, equal relative standard deviation (RSD = 15%, A and B) or equal standard deviation (SD = 5, C and D) were independently tested.