Figure 1
From: MELTING, a flexible platform to predict the melting temperatures of nucleic acids
Nearest-neighbor computation example. The duplex is composed of 3 patterns: two perfectly matching patterns (patterns 1 and 3) and one single mismatch (pattern 2). To compute the enthalpy and entropy of the perfectly matching patterns, MELTING 5 uses a single thermodynamic formula based on user-defined or default thermodynamic parameters. Other thermodynamic formula and parameters are used to compute the enthalpy and entropy of pattern 2.