Figure 3From: MELTING, a flexible platform to predict the melting temperatures of nucleic acidsSodium equivalence computation. MELTING uses a default formula to compute a sodium equivalent concentration (this figure illustrates an example of the formula from von Ahsen et al.[15]). This sodium equivalent concentration is then used by the sodium correction formula to modify the melting temperature calculated. This sodium correction can be included into approximative formulae or can be a separate formula applied during the Nearest-Neighbor computation. Users can overide the default formula and enter values for sodium, potassium or magnesium ion species.Back to article page