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Figure 2 | BMC Bioinformatics

Figure 2

From: Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

Figure 2

Schematic alignment of mRNA transcript variants from the ZNF419 gene. Numbers indicate the end positions of exons for variant 5. The red lines indicate the primer regions picked by Primer-BLAST. Note that several transcripts differ by 3 nucleotides due to use of slightly different splice sites even though they share same exons (i.e., variant 2 v.s. variant 1, variant 7 v.s. variant 6 and variant 4 v.s. variant 3). The graph is adapted from NCBI gene report (http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=full_report&list_uids=79744. Data accessed 11/02/2011).

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