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Table 7 Combination of TAGs with Primers — comparison of TAGs

From: EGNAS: an exhaustive DNA sequence design algorithm

 

TAGs

 

Fan et al.

EGNAS1*

Average ΔG

-0.3 ± 0.9

1.6 ± 0.5

Minimum ΔG

-2.7

0.4

GC content

45% - 50%

50%

L c

11

8

L hp

4

2

L sc

6

4

L sl

7

8

L ni

7

3

  1. Comparison of TAGs used by Fan et al.[8] and those generated by EGNAS. Intra- and interstrand properties. Molar free enthalpies (ΔG, kcal/mol) of the most stable hairpins. Averages ± standard deviations from 148 sequences.
  2. *GC ends and without {GGG;CCC;AAAAA;TTTTT}.