Figure 10
Symptoms caused by T3SS-2 mutants. Infection symptoms caused by the T3SS-2 mutants in Arabidopsis leaves. Wild type Salmonella or mutants in the SPI-2 encoded T3SS were infiltrated into Arabidopsis leaves; symptoms were analyzed during 5 DAI. a-d: Macroscopic observations of symptoms development from 0 to 5 DAI with (a): 10 mM MgCl2 (buffer control), (b): wild type 14028s strain, (c): ssa V−mutant, (d): ssa J−mutant. (e): Calculated average percentages of leaf surfaces showing infection symptoms. Five leaves per time point were photographed. Experiment was repeated five times. ∗p ≤ 0.05;∗∗p ≤ 0.005;∗∗∗p ≤ 0.0005 (Student’s t test).