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Table 3 Results of the experimental verification of predicted modules

From: An integrative computational approach to effectively guide experimental identification of regulatory elements in promoters

Predicted cis-regulatory modules

Fragments used to test the respective moduleb

Experimental round I

Experimental round II

1

Meme1a + TSS

301_1 (+), 48_1 (+)

134_1 (−), 156_4 (−), 212_2 (−)

2

NagC + BRCZ4 + TSS

272_1 (−)

134_1 (−), 134_2 (−), 212_2 (−)

3

(FNR + NagC) OR (FNR + NagC + TSS)

48_2 (−), 156_1 (−), 272_1 (−)

156_4 (−), 134_1 (−), 134_2 (−)

4

(TGIF + FNR + NagC) OR (TGIF + FNR + NagC + TSS)

301_1 (+), 48_1 (+)

134_3 (+), 212_1 (+)

5

Meme1a + HNF1

301_1 (+), 48_1 (+)

134_1 (−), 134_2

6

Meme1a + FNR + NagC

301_1 (+), 48_1 (+)

134_1 (−), 134_2 (−), 156_4 (−)

7

MEF2 + TGIF

301_2 (−)

 

8

BRCZ4 + HNF1

271_1 (−)

134_1 (−), 134_2 (−)

9

(RcsAB + MDScan3) OR (RcsAB + MDScan2)

48_2 (−)

134_1 (−), 134_2 (−)

10

(DME1 + RcsAB + Poly-(A)8 + TSS)

301_1 (+)

156_4 (−)

11

MEF2 + Meme4

156_2 (−), 272_2 (−), 48_2 (−)

 
  1. a Motif identical to tusp[6].
  2. b Fragments showing specific expression (+) support the respective regulatory module, fragments showing no- or unspecific expression (−) reject respective modules. After two experimental rounds module 4 is proved to be functional.