(a) Scatterplot of the normalized man vs. machine EDT. This is a comparison of the nEDT vs. cEDT values between fully manual and machine segmentation of 1086 nuclei, comprising 3720 gene markers. (b) Normalized EDT error as a function of position within the nucleus (0 = center, 1 edge) (c) Scatterplot of the cumulative man vs. machine EDT. (d) Cumulative EDT error as a function of position within the nucleus. FISH spots close to the periphery have larger error for cEDT, while those for nEDT are not position dependent. The red line is the median, the box extends to the 25th and 75th percentiles, the whiskers are the most extreme data points not considered outliers, outliers are plotted as red “+”s. Outliers above 0.3 not shown to improve visualization.