Table 4 Genes used in the qRT-PCR assays, and the sequence of the PCR primers used in the assays
From: The LO-BaFL method and ALS microarray expression analysis
Gene information | Gene role | Forward primer (5` to 3`)Reverse primer (3` to 5`) |
|---|---|---|
UBE2Z, NM_023079.3 | Reference gene | GCAGAGCATGTCTGGCATAG TTCTCCTTCTGCCAAAACAAA |
PGK1, NM_000291.3 | Reference gene | TGCATCTCCACTTGGCATTA TGGGATCTTGAAGAATGTATGC |
SRRM1, NM_005839.3 | Reference gene | GGAAATCCTTGGGTTTGAAGA GGCCACAGTTCTCCCATAAA |
COX4I1, NM_001861.2 | Reference gene | GGCACTGAAGGAGAAGGAGA GGGCCGTACACATAGTGCTT |
B2M, NM_004048 | DE gene determined by LO-BaFL/SAM | GATGAGTATGCCTGCCGTGTG CAATCCAAATGCGGCATCT |
ACTG1, NM_001614 | DE gene determined by LO-BaFL/SAM | AGAGGCTGGCAAGAACCAGTTGTT CAATGACGTGTTGCTGGGGCCT |
DYNLT1, NM_006519.1 | DE gene determined by TM4 analysis | CCAGCCTATGGCCTTTCTCCTTTTGT CAACGCAGGCTGCAGGTGAC |
SKIV2L2, NM_015360.4 | DE gene determined by TM4 analysis | TGCAGAAGGAATCACCAAAA ATGGGAGAACCAAATCCACA |
C12orf35, NM_018169.3 | DE gene determined by TM4 analysis | CGGGGAAACAAGGTATTTGA TTCACATCACAGTGGGCATT |
TARDBP, NM_007375.3 | DE gene determined by TM4 analysis | TTTGCTGCAGTTCTGTGTCC TCCATCTCAAAAGGTCAAAA |
ILKAP, NM_030768.2 | Cross-hybridizing gene with TARDBP | CACAGGAGTACACAAAACACAC TGCGGATAGGGCACTGAG |