Figure 1

Shabtai et al. Comparison of experiments performed on different dates. Two chemogenomic experiments performed using the same conditions (cantharidin, a protein phosphatase inhibitor) on different dates (a). These images show the extent of the differences between experiments that were performed using the same conditions. There is a difference in the scale of results (left experiment’s top value is ~22 representing a 10⁶ fold difference in abundance while right experiment’s top value is ~31 representing a 10⁹ fold difference in abundance). The lower results are the least affected genes, and include the majority of strains. These results vary in range of fitness defects between experiments, and are ignored because they are due to unmanageable differences between experiments, i.e. temperature perturbations. Despite the fact that the experiments were performed under the same conditions, the most sensitive deletion strains are not necessarily in the same ratio to each other nor are necessarily ranked in the same order (i.e. a strain can obtain the second highest fitness defect value in one experiment, and the third highest in another). Another representation of the differences between experiments is shown in image (b). The scatterplot shows an example of scores of two experiments performed using the same conditions. Top fitness defect scores are similar, though these strains are not in ranked the same for both experiments and have a different range of scores.