Figure 6From: ESTclean: a cleaning tool for next-gen transcriptome shotgun sequencingEvaluation method. Mapping results, A(E) and A(S), by GMAP for reads, E and S, cleaned by SeqClean and ESTclean respectively are evaluated to decide whether the reads are over- or under-trimmed. At the 5’ end, while SeqClean performs correct trimming, the read from ESTclean is under-trimmed as its 5’ end is not aligned to the genome. At the 3’ end, ESTclean over-trims while SeqClean under-trims because the latter has unaligned bases and the trimmed region of the former is real (aligned).Back to article page