From: QUAliFiER: An automated pipeline for quality assessment of gated flow cytometry data
Outlier function | Type | Use case |
---|---|---|
outlier.cutoff | threshold | 1. % of cells in WBC gate for RBC Lysis |
2. % of total events as boundary events | ||
3. Minimum total event count | ||
outlier.norm | robust normal | 1. Stability of MFI of a population vs time |
2. Consistency of gating (%) of a population | ||
3. High variability groups when measuring between–group variation (i.e. log(IQR) for boxplots) | ||
4. Individual outliers from residuals of robust regression (i.e. in xyplot) | ||
qoutlier | 1.5 × IQR | 1. Outliers within groups for boxplots |