Figure 1From: Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesisDistribution of co-evolving sites in Amy7C. Amy7C is shown in cartoon form, and the coevolving sites are shown in filled balls. The catalytic domain A, consisting of a closed eight-stranded parallel β sheet barrel (yellow) surrounded by eight α helices (blue), is circled in blue. Domain B that protrudes between third β-strand and third α-helix of domain A, is circled in green. The C-terminal β-sheet (red), domain C is circled in red. The sequences linking the domains are shown in gray. The dashed red line indicates the co-evolutionary relationship between each pair of co-evolving sites.Back to article page