Table 1 Consequence of non-conserved amino acid residues of XCE
ECE-1 | XCE | Change in Nature | Structural features | Subsite |
|---|---|---|---|---|
R145 | T146 | Basic to nucleophilic | No binding with carboxylate | S2’ |
W146 | Y147 | Aromaticity conserved | Volume of subsite is shrinked | S2’ |
F149 | I150 | Aromatic to aliphatic | Aromatic wall is absent | S1’ |
S150 | A151 | Nucleophilic to hydrophobic | No overall change | S2’ |
W153 | G154 | Aromatic to small aliphatic | Volume of subsite is shrinked | S2’ |
V565 | L570 | Hydrophobicity conserved | H-bond acceptor is conserved | NAYY |
F587 | L592 | Aromatic to aliphatic | Aromatic wall is absent | S1’ |
V603 | T608 | Hydrophobic to nucleophilic | No overall change | S1’ |
V604 | I609 | Hydrophobicity conserved | More interaction with the P1’ moiety | S1’ |
V713 | N718 | Hydrophobic to hydrophilic | Polarity at the bottom of subsite | S1’ |