Figure 1
From: Improved base-calling and quality scores for 454 sequencing based on a Hurdle Poisson model
The effect of preprocessing raw intensities. Raw intensities and flowgram values versus cycle number for a typical read, illustrating the effect of preprocessing. The colors represent the reference HPL. The preprocessing of raw intensities to flowgram values removes much noise. However, the raw intensities still contain additional information that can be used in the base-calling.