Detection efficiency. A. Distributions of the detection efficiency estimated from the quality index for each gene-sample site. B. Theoretical detection efficiency (at posterior probability cutoff h = 0.65) as a function of expected read depth, plotted for various values of the over-dispersion factor. C. Histograms of the quality index (QI) distribution in the three datasets. Overall, QI was highest in SC: 9.4±8.8 (median 6.6); second highest in BI: QI = 7.6 ± 5.6 (median 6.2); and lowest in BCM: QI = 5.5 ± 2.3 (median 5.0).