Figure 3

Functional enrichment and hierarchical clustering of putative miRNA target genes using GAGGLE framework in BRM. Hierarchical clustering and functional enrichment of the 199 mRNA transcripts putatively regulated by miRNAs as a result of processing through the miRNA workflow in Figure 2. These miRNA gene targets were significantly regulated by 30 uM nicotine exposure in developing zebrafish from 6–48 hours post fertilization and support the role of miRNAs in regulating nervous system development and function among other important biological processes.