TeraStitcher - A tool for fast automatic 3D-stitching of teravoxel-sized microscopy images

BMC Bioinformatics

Table 1 Comparison of displacements errors and quality measures among different datasets

Dataset	Tool	Error						Quality measure
		Max			Average			Min			Average
		V	H	D	V	H	D	V	H	D	V	H	D
	TeraStitcher	0	0	0	0	0	0	0.86	0.85	0.86	0.87	0.87	0.88
	Fiji	0	0	0	0	0	0		0.51			0.63
C. elegans worm^a	XuvStitch	0	0	0	0	0	0		0.72			0.78
	iStitch	0	0	0	0	0	0		0.94			0.95
	TeraStitcher	0	0	0	0	0	0	0.79	0.80	0.64	0.81	0.83	0.73
	Fiji	0	0	0	0	0	0		0.55			0.58
Neuron filaments^a	XuvStitch	0	0	0	0	0	0		0.67			0.76
	iStitch	0	0	1	0	0	0.3		0.90			0.92
	TeraStitcher	0	0	0	0	0	0	0.81	0.88	0.75	0.88	0.92	0.76
	Fiji	0	0	0	0	0	0		1			1
Fruit fly brain^b	XuvStitch	0	0	0	0	0	0		1			1
	iStitch	0	0	0	0	0	0		0.95			0.96
	TeraStitcher	1	0	1	0.5	0	0.5	0.71	0.68	0.53	0.73	0.7	0.61
Portion of mouse cerebellum^c (axons)	Fiji	0	0	0	0	0	0		0.72			0.73
Portion of mouse cerebellum^c (axons)	XuvStitch	0	0	0	0	0	0		0.2			0.31
	iStitch	7	1	0	3.5	0.5	0		0.95			0.96
	TeraStitcher	0	0	0	0	0	0	0.84	0.85	0.87	0.87	0.87	0.89
Portion of mouse brain^c (neurons and axons)	Fiji	0	0	0	0	0	0		0.77			0.80
Portion of mouse brain^c (neurons and axons)	XuvStitch	0	0	0	0	0	0		0.90			0.91
	iStitch	w.a.^d	w.a.	w.a.	w.a.	w.a.	w.a.		0.76			0.78
	TeraStitcher	0	0	0	0	0	0	0.76	0.77	0.6	0.8	0.84	0.75
Whole mouse cerebellum^c (Purkinje cells and axons)	Fiji	0	0	0	0	0	0		0.78			0.85
Whole mouse cerebellum^c (Purkinje cells and axons)	XuvStitch	3	3	2	0.8	0.8	1		0.75			0.87
	iStitch	1	0	0	0.2	0	0		0.92			0.94
	TeraStitcher	0	0	0	0	0	0	0.77	0.75	0.74	0.77	0.77	0.75
Whole mouse brain^c (neurons and axons)	Fiji	0	0	0	0	0	0		0.37			0.39
Whole mouse brain^c (neurons and axons)	XuvStitch	0	0	0	0	0	0		0.6			0.65
	iStitch	0	0	0	0	0	0		0.77			0.79

In this table we report a comparison of displacement errors (in voxels) and quality measures between TeraStitcher and the state-of-the art stitching tools when stitching Megavoxel-sized datasets.
^aPublicly available dataset downloaded from http://www.vaa3d.org.
^bPublicly available dataset downloaded from http://www.xuvtools.org.
^cCLSM dataset (courtesy of F.S. Pavone, L. Sacconi, L. Silvestri) available at http://www.iconfoundation.net.
^dWrong alignment.

ISSN: 1471-2105