Figure 4

Time-resolved clustering and individual tracking of a gene. A subset of essential mitotic genes (see Additional file 4) is depicted on each layer as nodes colored according to the associated knockdown effect, from yellow to blue (low to high impact). Grey represents 0 impact. Each layer corresponds to one phenotype. Clustering of gene knockdown profiles and gene tracking are highlighted for three individual time points: (a) t = 2 h; (b) t = 7 h; (c) t = 33 h. Dynamic clustering of genes on different layers reveals more dynamic changes for the "grape", "large" and "dynamic" phenotypes compared to "mitotic delay" or "polylobed", which tend to stay more constant, indicating that these phenotypes may be more stable compared to the previous ones. The gene lsm14a is tracked by node expansion (also indicated using arrows for "mitotic delay" and "grape"). Its silencing has a mild to more pronounced impact for the "mitotic delay" phenotype (a-b), while having no influence on phenotype "grape" in the beginning (a) and high towards the end (c), indicating a latent impact on the cell upon this particular knockdown that determines it to adopt "grape" morphology after stagnation during mitosis.