Figure 5

Similarity scoring of gene knockdown impact profiles. Scoring the overall impact of individual gene knockdowns on the prevalence of different phenotypes. We look at the span of one cell cycle, approximately 50 time points. Nodes correspond to gene knockdown events and are colored according to the scoring scale, as indicated (white-dark red, low-high). A set of 1067 essential mitotic genes is represented on each layer. One gene has the same position on all layers. Two alternative scoring schemes are presented: (a) averaging the values in the gene knockdown vector; (b) the lower bound of Wilson score confidence interval. A line chart of timeline evolution of knockdown values for each phenotype can be obtained by clicking on a particular node of interest, as shown for genes incenp and ranbp3, both of which display increasingly higher signal for the phenotype "polylobed" (green line) throughout the time course.