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Table 3 The main steps of the F2C2 algorithm

From: F2C2: a fast tool for the computation of flux coupling in genome-scale metabolic networks

Step

Rule

1.

Apply the preprocessing procedure shown in Table 1.

2.

Apply the feasibility rule using the feasible solutions obtained when solving the LP problems used in Step 1.

3.

Apply the TDC and TUC rules to determine trivially (un)coupled reactions.

4.

Identify fully coupled reversible reactions by solving the LP problems (2). This is not necessary if the kernel of the stoichiometric matrix is used in Step 1.

5.

Determine the dimension t of kern(SRev)and remove t independent fully reversible reactions and their coupled reactions. This step is optional since t is often small.

6.

Determine the flux coupling between (pseudo-)irreversible reactions by solving the LP problems (3) and (5).

7.

For each LP problem solved in Step 6, use the inference rules in Table 2 in combination with the feasibility rule.