From: F2C2: a fast tool for the computation of flux coupling in genome-scale metabolic networks
Step | Rule |
---|---|
1. | Apply the preprocessing procedure shown in Table 1. |
2. | Apply the feasibility rule using the feasible solutions obtained when solving the LP problems used in Step 1. |
3. | Apply the TDC and TUC rules to determine trivially (un)coupled reactions. |
4. | Identify fully coupled reversible reactions by solving the LP problems (2). This is not necessary if the kernel of the stoichiometric matrix is used in Step 1. |
5. | Determine the dimension t of kern(S∗Rev)and remove t independent fully reversible reactions and their coupled reactions. This step is optional since t is often small. |
6. | Determine the flux coupling between (pseudo-)irreversible reactions by solving the LP problems (3) and (5). |
7. | For each LP problem solved in Step 6, use the inference rules in Table 2 in combination with the feasibility rule. |