Comparison with Bl method. Comparison of Base errors per cycle for the BM-BC method (right panel) and the B-I method (left panel) in Bravo and Irizarry (2010) for a random subset of 50,000 colonies. The error rate of base calls is about 4.9% for the BM-BC and about 8.0% for the B-I method. The G-T substitution error curve (shown by a turquoise green solid line) and the A-C substitution curve (shown by a blue dotted line) dominates the other pairwise substitution rate in both the methods. However, clearly, the curves in the BM-BC are lower both in the absolute scales and in the rate of increase with cycles.