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Table 3 Summary of potential problems encountered during analysis of SID-MRM-MS data that often require manual identification or re-integration and their effect on the precision and accuracy of quantification.

From: Statistical characterization of multiple-reaction monitoring mass spectrometry (MRM-MS) assays for quantitative proteomics

Data Issue Impact on Quantification
Poor chromatographic peak shape Imprecise and inaccurate area assessment
Chromatographic peak too narrow (<6 points across) Imprecise and inaccurate area assessment
Detector saturation Inaccurate peak area assessment
Inconsistent integration between analyte and SIS peptides Imprecise and inaccurate peak area assessment
Interference in analyte or SIS signals Inaccurate peak area assessment