Table 3 Summary of potential problems encountered during analysis of SID-MRM-MS data that often require manual identification or re-integration and their effect on the precision and accuracy of quantification.
Data Issue | Impact on Quantification |
|---|---|
Poor chromatographic peak shape | Imprecise and inaccurate area assessment |
Chromatographic peak too narrow (<6 points across) | Imprecise and inaccurate area assessment |
Detector saturation | Inaccurate peak area assessment |
Inconsistent integration between analyte and SIS peptides | Imprecise and inaccurate peak area assessment |
Interference in analyte or SIS signals | Inaccurate peak area assessment |