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Table 5 Summary of Results for Studies I, II, and III (combined results for process replicates a, b, c) for each peptide across sites for inter-site CV, intra-site CV, linear slope and % recovery.

From: Statistical characterization of multiple-reaction monitoring mass spectrometry (MRM-MS) assays for quantitative proteomics

Signature Peptide

Study I

Study II

Study IIIa

 

Inter- site CV

Intra- site CV

Linear Slope

% Recov.

Inter- site CV

Intra- site CV

Linear Slope

% Recov.

Inter- site CV

Intra- site CV

Linear Slope

% Recov.

APR-AGL

9.20%

3.9-11.2%

1.157

114.5

13.10%

2.0-7.8%

0.575

57.5

13.70%

7.3-45.2%

0.738

79.4

CRP-ESD

5.90%

2.2-5.9%

1.124

118.4

10.50%

3.1-8.4%

0.573

61.4

16.70%

8.5-18.1%

0.439

48.9

CRP-GYS

5.40%

1.4-10.2%

1.324

140.5

5.60%

1.2-6.4%

0.546

56

18.50%

6.6-35.0%

0.159

18.5

HRP-SSD

14.10%

4.0-8.9%

1.198

120.4

5.50%

4.6-7.3%

0.794

82.3

21.90%

8.4-21.4%

0.43

45.7

LEP-IND

12.50%

2.9-10.3%

1.163

119.1

29.50%

2.6-15.3%

0.152

14.9

50.40%

11.7-54.9%

0.242

25.6

MBP-HGF

4.30%

1.7-6.3%

1.161

118.6

9.30%

1.5-7.8%

0.758

77.3

21.80%

7.4-32.8%

0.238

23.8

MBP-YLA

5.10%

2.1-9.3%

1.275

130.3

4.10%

1.5-14.1%

0.806

83.8

N/A

N/A%

N/A

> 1.0

MYO-LFT

4.90%

1.6-5.7%

1.518

154.4

3.80%

2.0-6.3%

1.012

101.3

23.10%

8.9-21.6%

0.504

60.4

PSA-IVG

6.90%

1.3-14.7%

1.658

165.4

5.50%

2.0-11.2%

0.848

81.9

17.20%

7.6-13.7%

0.587

58

PSA-LSE

8.90%

1.2-6.9%

1.098

111.4

5.30%

2.0-4.6%

1.524

151.3

10.30%

7.6-13.7%

0.918

92.7

  1. The CVs increase in distribution between studies I, II, and III as expected with increasing complexity of the three studies. Adapted from Addona, et. al., Nature Biotechnology, 27(7):633-41 (25).