Skip to main content

Table 5 Summary of Results for Studies I, II, and III (combined results for process replicates a, b, c) for each peptide across sites for inter-site CV, intra-site CV, linear slope and % recovery.

From: Statistical characterization of multiple-reaction monitoring mass spectrometry (MRM-MS) assays for quantitative proteomics

Signature Peptide Study I Study II Study IIIa
  Inter- site CV Intra- site CV Linear Slope % Recov. Inter- site CV Intra- site CV Linear Slope % Recov. Inter- site CV Intra- site CV Linear Slope % Recov.
APR-AGL 9.20% 3.9-11.2% 1.157 114.5 13.10% 2.0-7.8% 0.575 57.5 13.70% 7.3-45.2% 0.738 79.4
CRP-ESD 5.90% 2.2-5.9% 1.124 118.4 10.50% 3.1-8.4% 0.573 61.4 16.70% 8.5-18.1% 0.439 48.9
CRP-GYS 5.40% 1.4-10.2% 1.324 140.5 5.60% 1.2-6.4% 0.546 56 18.50% 6.6-35.0% 0.159 18.5
HRP-SSD 14.10% 4.0-8.9% 1.198 120.4 5.50% 4.6-7.3% 0.794 82.3 21.90% 8.4-21.4% 0.43 45.7
LEP-IND 12.50% 2.9-10.3% 1.163 119.1 29.50% 2.6-15.3% 0.152 14.9 50.40% 11.7-54.9% 0.242 25.6
MBP-HGF 4.30% 1.7-6.3% 1.161 118.6 9.30% 1.5-7.8% 0.758 77.3 21.80% 7.4-32.8% 0.238 23.8
MBP-YLA 5.10% 2.1-9.3% 1.275 130.3 4.10% 1.5-14.1% 0.806 83.8 N/A N/A% N/A > 1.0
MYO-LFT 4.90% 1.6-5.7% 1.518 154.4 3.80% 2.0-6.3% 1.012 101.3 23.10% 8.9-21.6% 0.504 60.4
PSA-IVG 6.90% 1.3-14.7% 1.658 165.4 5.50% 2.0-11.2% 0.848 81.9 17.20% 7.6-13.7% 0.587 58
PSA-LSE 8.90% 1.2-6.9% 1.098 111.4 5.30% 2.0-4.6% 1.524 151.3 10.30% 7.6-13.7% 0.918 92.7
  1. The CVs increase in distribution between studies I, II, and III as expected with increasing complexity of the three studies. Adapted from Addona, et. al., Nature Biotechnology, 27(7):633-41 (25).
\