Figure 1

Time-series image analysis workflow for the study of muscle remodeling in Drosophila metamorphosis. (a) Upon multi-location 3D time-lapse microscopy, image data belonging to the same location are concatenated using the TLM-Converter and converted into open source ICS files. (b) The TLM-2D-Explorer produces maximum intensity projections (MIP) of 3D stacks for the rapid visual inspection of time-lapse images. Multiple MIP time-lapse datasets can be displayed side-by-side to assist in the identification of phenotypic abnormalities. Developmental reference points, such as PPT, can be used for the temporal alignment of multiple datasets. (c) To examine the morphological dynamics of individual muscle fibers in 3D, the TLM-3D-Segmenter performs semi-automated segmentation based on level sets and Bayesian surface evolution.