Figure 5

Segmentation facilitates visualization and quantification of muscle remodeling. (a) Selected dorsal views derived from MIPs of a pupal abdomen where muscles are labeled using Grasp65-GFP (green). Arrows indicate two segmented persistent muscles. The scale bar in the top panel represents 100 μm and applies to all other panels. (b) Iso-surface rendering of the two segmented muscles. Syncytial nuclei are rendered in red. (c, d) Segmentation permits the measurement of dynamic features, such as volume (c), diameter and the axis of inertia.