Functional relevance of dynamic properties of Dimeric NADP-dependent Isocitrate Dehydrogenases

BMC Bioinformatics

Table 2 Active site residues.

	EcIDH	BsIDH	ApIDH	BpIDH	MtIDH1	HcIDH	YmIDH	PmIDH	TmIDH	TtIDH	AvIDH	CgIDH
Subfamily	I	I	I	I	II	II	II	II	II	IV	M	M
Phos loop start	T105	T96	T112	T107	T78	T78	T77	T78	T77	T90	S86	S85
Phos loop end	S113	S104	S120	S115	S95	S95	S94	S95	S94	S98	S132	S130
Isocitrate Binding	N115	N106	N122	N117	N97	N97	N96	N97	N96	N100	N135	N133
Isocitrate Binding	R119	R110	R126	R121	R101	R101	R100	R101	R100	R104	R139	R137
Isocitrate Binding	R129	R119	R136	R131	R110	R110	R109	R110	R109	R114	R145	R143
Isocitrate Binding	R153	R143	R159	R155	R133	R133	R132	R133	R132	R138	R547	R543
Isocitrate Binding	Y160	Y150	Y166	Y162	Y140	Y140	Y138	Y140	Y139	Y143	Y420	Y416
Active site.	K230'	K220'	K233'	K232'	K213'	K213'	K212'	K212'	K208'	K191'	K255	K253
Active site.	D283'	D286'	D287'	D285'	D252'	D252'	D252'	D252'	D247'	D224'	D350	D348
Metal binding	D307'	D310'	D311'	D309'	D276'	D276'	D275'	D275'	D270'	D248'	D548	D544
N-loop start.	G340	G345	G344	G342	G311	G311	G310	G310	G304	G281	G583	G579
NADP binding	K344	K349	K348	K346	R315	R315	R314	R314	R308	K285	K588	K584
NADP binding	Y345	Y350	Y349	Y347	H316	H316	H315	H315	H309	Y286	H589	H585
N-loop end	G347	G352	G351	G349	G323	G323	G322	G322	G316	G288	G597	N593
N-loop extension	N352	N357	N356	N354	N329	N329	N328	N328	N322	N293	D602	D598

Active site residues in Isocitrate Dehydrogenases. The residues in S1, S2 and S4 align properly in structural alignment. Functionally monomeric IDHs (type M) are also included for comparison. In monomeric IDHs, the respective residues don't appear in the same sequence. They do not have a Phos loop. Serine residues (such as S86 in AvIDH) play a similar role to threonines in dimeric IDHs and are indicated in italic font. N-loop refers to the NADP binding loop.

ISSN: 1471-2105